LOCUS       V005233                 4999 bp    DNA     circular SYN 18-DEC-2018
DEFINITION  Exported.
ACCESSION   V005233
VERSION     V005233
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
            .
REFERENCE   1  (bases 1 to 4999)
  AUTHORS   Toda, H., Itoh, N.
  TITLE     Development of novel E. coli-Kocuria shuttle vector using cryptic
            plasmid pKPAL3 from Kocuria palustris IPUFS-1 and utilization for
            producing enantiopure (S)-styrene oxide
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4999)
  AUTHORS   Itoh N, Toda, H.
  TITLE     Direct Submission
  JOURNAL   Submitted (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural
            University, Biotechnology Research Center; 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http://www.pu-toyama.ac.jp/BR/itoh/
REFERENCE   3  (bases 1 to 4999)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 4999)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName:
            "Unpublished"
            SGRef: number: 2; type: "Journal Article"; journalName: "Submitted
            (22-AUG-2017) Contact:Nobuya Itoh Toyama Prefectural University,
            Biotechnology Research Center"; volume: " 5180 Kurokawa, Imizu,
            Toyama 939-0398, Japan URL :http"; pages:
            "//www.pu-toyama.ac.jp/BR/itoh"
            SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..4999
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    39..60
                     /label="CAP binding site"
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        75..105
                     /label="lac promoter"
                     /note="promoter for the E. coli lac operon"
     protein_bind    113..129
                     /label="lac operator"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be
                     relieved by adding lactose or
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     137..153
                     /label="M13 rev"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     misc_feature    163..219
                     /label="MCS"
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(223..239)
                     /label="M13 fwd"
                     /note="common sequencing primer, one of multiple similar
                     variants"
     rep_origin      452..907
                     /label="f1 ori"
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        1189..1293
                     /label="AmpR promoter"
     CDS             1294..2151
                     /label="AmpR"
                     /note="beta-lactamase"
     rep_origin      2325..2913
                     /direction=RIGHT
                     /label="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
                     replication"
     CDS             3141..3947
                     /gene="tsnR"
                     /label="23S rRNA (adenosine(1067)-2'-O)-methyltransferase"
                     /note="23S rRNA (adenosine(1067)-2'-O)-methyltransferase
                     from Streptomyces azureus. Accession#: P18644"
     rep_origin      3996..4999
                     /label="pKPAL3-derived plasmid replication origin"
                     /note="pKPAL3-derived plasmid replication origin"
ORIGIN
        1 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
       61 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
      121 ggataacaat ttcacacagg aaacagctat gaccatgatt acgaattcga gctcggtacc
      181 cggggatcct ctagagtcga cctgcaggca tgcaagcttg gcactggccg tcgttttaca
      241 acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
      301 tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
      361 cagcctgaat ggcgaatggc gcctgatgcg gtattttctc cttacgcatc tgtgcggtat
      421 ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct gtagcggcgc attaagcgcg
      481 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
      541 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
      601 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
      661 cttgatttgg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
      721 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
      781 aaccctatct cgggctattc ttttgattta taagggattt tgccgatttc ggcctattgg
      841 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
      901 acaattttat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagccc
      961 cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc ggcatccgct
     1021 tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc accgtcatca
     1081 ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     1141 ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     1201 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     1261 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     1321 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1381 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1441 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1501 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1561 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1621 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1681 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1741 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1801 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1861 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1921 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1981 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     2041 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     2101 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     2161 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     2221 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     2281 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     2341 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2401 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2461 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2521 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2581 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2641 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2701 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2761 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2821 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2881 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttagatcc
     2941 tgaacttttg ctttgccacg gaacggtctg cgttgtcggg aagatgcgtg atctgatcct
     3001 tcaactcagc aaaagttcga tttattcaac aaagccacgt tgtgtctcaa aatctctgat
     3061 gttacattgc acaagataaa aatatatcat catgaacaat aaaactgtct gcttacataa
     3121 cccgttggag gaggctccaa atgaccgagc tggacaccat cgcgaacccc tccgatcccg
     3181 ccgtgcagcg gatcatcgac gtgaccaaac cctcccgctc gaacatcaag accaccctca
     3241 tcgaggacgt cgagcccctg atgcactcca tcgccgcggg cgtggagttc attgaggtgt
     3301 acgggtccga ctcgtccccg ttcccctcgg aactgctcga tctgtgcggc cgccagaaca
     3361 tccccgtgcg gctgatcgac tcctccatcg tgaaccagct cttcaagggc gagcgcaaag
     3421 ccaagacctt cgggatcgcc cgcgtgcccc gccccgcgcg cttcggcgac attgcctccc
     3481 gccgcggcga cgtggtggtg ctggacggcg tgaaaatcgt gggcaacatc ggcgcgatcg
     3541 tgcgcacgtc cctcgccctc ggcgcctccg gcatcatcct ggtggactcc gacattacgt
     3601 ccatcgcgga ccgccgcctg cagcgcgcct cccgcgggta tgtgttctcc ctgcccgtgg
     3661 tgctgtccgg gcgcgaggag gcgatcgcct tcatccgcga ctccggcatg cagctgatga
     3721 ccctgaaggc cgacggcgac atctcggtga aggagctggg cgacaacccc gaccgcctgg
     3781 cgctgctgtt cggctccgag aagggcggcc cctccgacct gtttgaggag gcctcctccg
     3841 cgtccgtgtc catccccatg atgtcccaga ccgagtccct gaacgtgtcc gtgtccctgg
     3901 gcatcgcgct gcacgagcgc atcgaccgca acctcgccgc gaaccgctga gcgcctctgt
     3961 tcctcggacg ctcggttgct cgacctcgat tcgtcagatc tttcgctcgt gctgttcttt
     4021 gaggccctgc cctccgcctt gctgccttcg tgctgtgctc gggatctcga gcgcaacctc
     4081 cgaccagcct gggagggaag gagggagcgg agggcagggc gatgccttgg acgccccgag
     4141 gacacccggg cggggtgtgg tggggacgtt caaggcatcg tcaatcggtg gtgcgggacc
     4201 agcccggtcg gtcggtcggt cgcatcggtt ggccaccgtt ggtcacgcac cgtgaggggt
     4261 gcgtggggtg gctgacaacg gctcagggcg ttactcctgt gcagtggcgg catgtgcgtg
     4321 cagctctggt cacacgctcg gggcagggct gctgtctccc gagggcggct gtagggcttg
     4381 gcactccttc gctgtccggg gattgctcga cccggtgacg gcccctggct gcacgctcgg
     4441 caaaagaacc ggctctgacc cgggaggggc cgcgaaggca gtgcctcaca agcggctggc
     4501 gatgttggtt cacggggctt cgcatcccga cgccagggtg ttccgatcgc tggcgggtcc
     4561 agttgcaggc cgttggcgcg gcggtagact ggtctcggta tttactacag acctcatggt
     4621 aggcccccgt tggcgcgggg gcctaccgca tacctagggg ggcgtgtcgt cagacacgcc
     4681 ccctaggtgt tgtggggcat gaggttcttg tcaccagcgg cgcgctgaga tgaggcgagc
     4741 gggctcctcg tcacaggatg gaagttcctc aacaagccat ccacggaaga cagagcccgc
     4801 tcatgcccca cgctaagcga agttttctac gatgcagtag atcccgcagg gtacgtaagt
     4861 agaacaattc agcacatcgc cagtcatgcc ttcaccccta gtgaggtgcg tcagctttta
     4921 gaaacctccg gcttcaagaa tattgcaatg tatgggggtt tttctttcgg ccccctaact
     4981 gattcaagtt tcgccatgg
//
